real-timeRT-PCR相关论文
目的:应用实时荧光RT-PCR和半巢式RT-PCR方法检测和分析牡蛎中诺如病毒的基因特征。方法:应用实时荧光RT-PCR和半巢式RT-PCR方法，对......
A Real-time Reverse Transcription PCR Assay for Detection of Cucumber mosaic virus in Individual Pea
Cucumber mosaic virus(CMV) is transmitted in a nonpersistent manner by peach aphid (Myzus persicae),leading to many crop......
Detection of Cyprinid Herpesvirus 2 Genome from Peripheral Blood Cells of Silver Crucian Carp Carass
Epidemics caused by Cyprinid herpesvirus 2(CyHV-2)in domestic cyprinid species have been reported in both European and A......
Suppression subtractive hybridization and real time RT-PCR reveal differentially expressed immune-re
Since 2011, Cyprinid herpesvirus 2 (CyHV-2) has been associated with the mortality of cultured crucian carp Carassius au......
Cloning, characterization and expression analysis of Nucleoside Phosphorylase 5a (PNP 5a) from Cruci
Purine nucleoside phosphorylase 5a (PNP5a) is a ubiquitous enzyme of purine metabolism, which catalyses the reversible p......
目的 表观遗传学研究显示miRNA 可能与肿瘤发生发展过程重起着重要作用，我们前期研究示在食管鳞癌中高表达，而且随着TNM 分期和局部......
[Gly14]-humanin abolished Aβ31-35-induced neurotoxicity by activating STAT3 transcription factor pat
Objective Humanin (HN) is a 24-amino acid bioactive peptide abolishing neuronal cell death induced by various familial A......
Objectives: Dysfunctional Dopamine D2 Receptor (DRD2) may play a role in the pathophysiology of TS.This study aimed to e......
Curcumae Rhizoma (CR) has been used in traditional Chinese medicine for thousands of years as an anti-blood stasis and p......
Functional Analysis of Stress-related genes SML Ⅱ in Salvia miltiorrhiza by quantitative competitive
Background:Salvia miltiorrhiza Bunge is a perennial herb in the Labiatae family.Its dry root or rhizome,designated as Da......
The cell adhesion molecules CD44v6 and integrin-β 1 are associated with the progression and metastasis of cancer.A nove......
The Spatio-tempral Expression Difference of ADA, Lectin, Serpin Gene in Aedes albopictus Salivary Gl
The saliva of mosquito is enriched with molecules that display anti-hemostatics,anti-inflammatory and immunomodulatory f......
We previously demonstrated that serine protease inhibitor BmSPI could strongly inhibit subtilisin A.In order to characte......
目的 分析2018至2019年广州市区某院甲型流感病毒的流行特征,评估胶体金标记免疫层析法和Real?time RT?PCR法在甲型流感病毒筛查中......
目的 分析郑州市疾控中心病毒实验室检测并确诊的12例人感染H7N9禽流感病例及6年禽流感外环境监测的实验室检测结果,为本地区今后......
通过已建立的兔TLR-2、4mRNA表达水平的SYBR GreenⅠ荧光定量RT-PCR(RRT-PCR)方法,对斯氏艾美尔球虫(E.stiedai)感染后的裂殖生殖......
为了建立鉴别检测小反刍兽疫疫苗毒与野毒的快速分子生物学检测方法，通过对自行测序的疫苗毒基因组序列及GenBank中登录的野毒基因......
目的:评估哈尔滨中东呼吸综合征(MERS)疫情发生的可能性,对可疑病例进行病原学检测.方法:采用风险矩阵法对哈尔滨市发生MERS疫情的......
目的了解惠州市手足口病肠道病毒的感染情况,为制定预防和控制肠道病毒感染策略提供依据。方法采集2011年7—12月我院送检的疑似手......
Detection of carcinoembryonic antigen mRNA in peritoneal washes from gastric cancer patients and its
AIM: To establish a more sensitive method for detection of free cancer cells in peritoneal washes from gastric cancer pa......
目的:探讨西尼罗病毒(West Nile virus,WNV)在新疆伊犁地区马群中的流行状况.方法:采用一步法实时定量逆转录聚合酶链反应(Real-ti......
目的:探讨西尼罗病毒(West Nile virus,WNV)在新疆伊犁地区马群中的流行状况.方法:采用一步法实时定量逆转录聚合酶链反应(Real-ti......
鸡传染性法氏囊病病毒（IBDV）超强毒株的出现给该病的防控带来了新的困难,有效鉴别IBDV超强毒株对IBD的防控有着积极意义。根据IBDV V......
目的为确定引起腹泻的病原体，对腹泻患者标本进行诺如病毒核酸检测。方法应用实时荧光RT—PCR法对5份感染性腹泻患者粪便标本进行GI......
目的建立基于AllGlo探针Real—time RT—PCR技术检测发热伴血小板减少综合征病毒（SFTSV）的方法。方法根据SFTSV基因组S片段的相对保......
旨在评价H1N1猪流感病毒感染猪血管内皮细胞（PUvEc）后，肽基脯氨酰异构酶A（Ppia）、B-肌动蛋白（Actb）、核糖体蛋白L4（Rpl4）、酪氨酸3／色氨酸5-......
目的探索安宫牛黄丸对小鼠肝脏细胞色素P450酶系亚型mRNA表达的影响。方法 将健康雄性小鼠随机分为3组:空白对照组、安宫牛黄丸低......
目的对崇左市2009年流感实验室检测结果进行分析,了解2009年崇左市流感病原学特征,为流感的实验室诊断提供参考。方法收集2009年9......
动物体细胞重编程为诱导多能干细胞(iPS)是目前干细胞生物学研究的热点。文中重点对山羊体细胞重编程过程中端粒酶(TERT)基因的相......
背景：辛伐他汀对正常大鼠骨代谢及骨髓基质干细胞增殖分化影响的报道目前不多。目的：观察辛伐他汀体内给药对大鼠骨量和骨髓基质干细......
自2010年4月以来，在我国东南地区省份的部分鸭场中先后爆发了一种急性传染病，主要引起蛋鸭产蛋急剧下降。鸭群患病后4～5d左右产蛋率可......
拟南芥GLABRA2（AtGL2）在决定拟南芥叶毛和根毛细胞的分化中起重要作用。通过同源序列查询从棉花的EST库中找到4个与AtGL2高度同源的......
